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KMID : 1146920220520060749
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Journal of Pharmaceutical Investigation
2022 Volume.52 No. 6 p.749 ~ p.764
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Catalase-mimicking synthetic nano-enzymes can reduce lipopolysaccharide-induced reactive oxygen generation and promote rapid detection of hydrogen peroxide and L-cysteine
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Revuri Vishnu
Mondal Jagannath
Mohapatra Adityanarayan
Rajendrakumar Santhosh Kalash
Surwase Sachin S.
Park In-Kyu
Park Joo-Ho
Lee Yong-Kyu
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Abstract
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Purpose: Here, we present the fabrication of a novel molybdenum-based, synthetic blue nano-enzyme (Synz) as an in situ oxygenic catalase mimic for the reduction of H2O2 and the detection of hydrogen peroxide (H2O2) and L-cysteine (L-Cys).
Methods: Molybdenum-based synthetic nano-enzymes (Synz) were fabricated by the flash freeze?heating method. Field emission scanning electron microscopy, energy-dispersive X-ray spectrometry, X-ray diffraction analysis, X-ray photoelectron spectroscopy (HP-XPS) and Fourier transform infrared spectroscopy were used for the physicochemical characterization of the developed Synz. Ultraviolet?visible (UV?Vis) absorption spectra were used to detect both H2O2 and L-Cys. Digital photographs were taken to visualize the colour changes in Synz-coated paper incubated with both H2O2 and L-Cys.
Results: Synz was used as a colorimetric biosensor for the detection of both H2O2 and L-Cys. Furthermore, co-incubation with lipopolysaccharide and Synz reduced the levels of both H2O2 and reactive oxygen species in J77A4 macrophage cells. Additionally, the incubation of H2O2 on Synz-coated cellulose filter paper led to a substantial change in the paper’s colour from blue to white with a low H2O2 detection limit of 10 μM and, on the contrary, retained the blue colour with the addition of an L-Cys?+?H2O2 solution with L-Cys concentration detection limit of 70 μM.
Conclusion: Collectively, our results show Synz to be a promising catalase-mimicking biomaterial that can alleviate H2O2 and be used to develop a low-cost colorimetric paper biosensor for prognostic or theranostic applications.
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KEYWORD
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Molybdenum, Synthetic enzyme, Paper biosensor, Hydrogen peroxide, L-cysteine
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